Yioulaki was born on 27 March 1938 in Athens. She studied at the Dramatic School at the National Theatre of Greece. Her first appearance in the theater was in 1959 with Dinos Iliopoulos in the work I kiria tou kiriou (Η κυρία του κυρίου).
Oi kyries tis avlis (Greek: Οι κυρίες της αυλής/ English: Ladies of the Courtyard) is a 1966 Greek film based on the theatrical play To ekto patoma (Το έκτο πάτωμα = The Next Step).