Archaeoglobus profundus (Burggraf et al. 1990) is a hyperthermophilic archaeon in the euryarchaeal class Archaeoglobi, which is currently represented by the single family Archaeoglobaceae, containing six validly named species and two…
How microbial communities change over time in response to the environment is poorly understood. Previously a six-year time series of 16S rRNA V6 data from the Western English Channel demonstrated robust seasonal structure within the…
MCF-7 cells were maintained in DMEM supplemented with 10% calf serum. The stable PAD2-depleted MCF-7 cell line was described previously [12] and maintained in the medium containing 1 µg/ml puromycin (Sigma).
In molecular biology, G-quadruplex secondary structures (G4) are formed in nucleic acids by sequences that are rich in guanine. [2 ] They are helical in shape and contain guanine tetrads that can form from one, [3 ] two [4 ] or four strands…
Novel high-throughput sequencing methods outperform earlier approaches in terms of resolution and magnitude. They enable identification and relative quantification of community members and offer new insights into fungal community ecology.
Dilated cardiomyopathy (DCM) is defined by progressive functional and structural changes. We performed RNA-seq at different stages of disease to define molecular signaling in the progression from pre-DCM hearts to DCM and overt heart…
Given the advent of massively parallel DNA sequencing, human microbiome is analyzed comprehensively by metagenomic approaches. However, the inter- and intra-individual variability and stability of the human microbiome remain poorly…
Caenorhabditis elegans (/ ˌ s iː n oʊ r æ b ˈ d aɪ t ə s ˈ ɛ l ə ɡ æ n s/ [6 ]) is a free-living transparent nematode about 1 mm in length that lives in temperate soil environments. [7 ] It is the type species of its genus. [8 ] The name is…
Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers.