Self-renewal and differentiation of spermatogonial stem cells (SSCs) provide the foundation for testis homeostasis, yet mechanisms that control their functions in mammals are poorly defined.
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Cells were stimulated for the indicated times with LPS (Tlr4) (100 ng/ml), Pam3CSK4 (Tlr2/1) (100 nM), Pam2CSK4 (Tlr2/6) (100 ng/ml), PolyI:C (Tlr3) (25 μg/ml) or poly(dA-dT) (5 μg/ml) which was transfected with lipofectamine 2000 from…