The 2001 Tercera División play-offs to Segunda División B from Tercera División (Promotion play-offs) were the final playoffs for the promotion from 2000–01 Tercera División to 2001–02 Segunda División B.
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The last five teams in each group are relegated to the Tercera Federación; in addition, the four worst teams classified 13th in their group play in the relegation play-offs, to define the last two relegation places.